Image-processing in python: input of photos with many sperm/egg cells and need to find the area of the cells -


i working on project bio lab, has thousands of images of cells (sperm , egg) taken microscope. job automate process count cells in each image , record area. have done research sobel/canny edge detection, not sure best way pick out/separate cells. coding in python 2.7 , using pil/maybe opencv. advice start?

the following picture has had sobel edge detection run on it. other images processing similar. can see, there still lot of shapes in background.

sobel edge detected image

enter image description here

original image

here picture before sobel detection


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